A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders
نویسندگان
چکیده
A common challenge when studying rare diseases or medical conditions is the limited number of patients, usually resulting in long inclusion periods as well unequal sampling and storage conditions. The main purpose this study was to demonstrate challenges comparing samples subject different preanalytical We performed a global (commonly referred “untargeted”) liquid chromatography-high resolution mass spectrometry metabolomics analysis blood from cases sudden infant death syndrome controls stored dried spots on chemical-free filter card for 15 years at room temperature compared with same whole −80°C before preparing new using chemically treated card. Principal component plots distinctly separated based type storage, but not versus controls. Note that, 1263 out 5161 642 1587 metabolite features detected positive negative ionization mode, respectively, were found have significant 2-fold changes amounts corresponding demonstrates that spot metabolome largely affected by factors. This emphasizes importance thoroughly addressing factors during design interpretation, enabling identification real, biological differences between sample groups whilst preventing other random variation be falsely interpreted results.
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ژورنال
عنوان ژورنال: Analytical science advances
سال: 2023
ISSN: ['2628-5452']
DOI: https://doi.org/10.1002/ansa.202300010